Detection of UGT1A1*28 Polymorphism Using Fragment Analysis

VernacularTitle:一种快速检测UGT1A1*28基因多态性的方法
Author: HUANG YING ¹ ; SU JIAN ; HUANG XIAOSUI ; LU DANXIA ; XIE ZHI ; YANG SUQING ; GUO WEIBANG ; LV ZHIYI ; WU HONGSUI ; ZHANG XUCHAO
Author Information

1. 510080广州,广东省人民医院,广东省医学科学院医学研究部,广东省人民医院肺癌研究所转化医学重点实验室
Keywords: UGT1A1; Fragment analysis; Polymorphism
From: Chinese Journal of Lung Cancer 2017;20(12):817-821
CountryChina
Language:Chinese
Abstract: Background and objective Uridine-diphosphoglucuronosyl transferase 1A1 (UGT1A1),UGT1A1 *28 polymorphism can reduce UGT1A1 enzymatic activity,which may lead to severe toxicities in patients who receive irinotecan.This study tries to build a fragment analysis method to detect UGT1A1 *28 polymorphism.Methods A total of 286 blood specimens from the lung cancer patients who were hospitalized in Guangdong General Hospital between April 2014 to May 2015 were detected UGT1A1*28 polymorphism by fragment analysis method.Results Comparing with Sanger sequencing,precision and accuracy of the fragment analysis method were 100％.Of the 286 patients,236 (82.5％ harbored TA6/6 genotype,48 (16.8％) TA 6/7 genotype and 2 (0.7％) TA7/7 genotype.Conclusion Our data suggest hat the fragment analysis method is robust for detecting UGT1A1 *28 polymorphism in clinical practice.It's simple,time-saving,and easy-to-carry.