EGFR and KRAS Gene Mutations in 754 Patients with Resectable Stage Ⅰ-Ⅲa Non-small Cell Lung Cancer and Its Clinical Significance
10.3779/j.issn.1009-3419.2017.09.05
- VernacularTitle:754例Ⅰ期-Ⅲa期可手术切除非小细胞肺癌患者EGFR和KRAS基因突变状态及其临床意义
- Author:
ZHAO JING
1
;
GAO JIE
;
GUO LIPING
;
HU XIAOXU
;
LIU QI
;
ZHAO JINYIN
;
LIU LICHENG
;
JIANG JUN
;
WANG MENGZHAO
;
LIANG ZHIYONG
;
XU YAN
;
CHEN MINJIANG
;
ZHANG LI
;
LI LONGYUN
;
ZHONG WEI
Author Information
1. 100730北京,中国医学科学院,北京协和医学院,北京协和医院呼吸科
- Keywords:
Lung neoplasms;
Epidermal growth factor receptor gene;
Kirsten rat sarcoma viral oncogene;
Resectable samples
- From:
Chinese Journal of Lung Cancer
2017;20(9):617-622
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Epidermal growth factor receptor (EGFR) and KRAS gene are important driver genes of non-small cell lung cancer (NSCLC).The studies are mainly focused on detection ofEGFR gene for advanced NSCLC,and the mutation feature of EGFR and KRAS gene in early NSCLC tissue is unknown.This study aims to investigate the mutations of EGFR and KRAS gene in NSCLC,and the relationship between the genotype and clinicopathologic features.Methods The hotspot mutations in EGFR and KRAS gene in 754 tissue samples of stage Ⅰ-Ⅲa NSCLC from Department of Pathology,Peking Union Medical College Hospital were detected by modified amplification refractory mutation system (ARMS) real-time PCR kit,and analyzed their correlation with clinical variables.Results The hotspot mutation rates in EGFR and KRAS were 34.5％ and 13.1％ respectively,and there were EGFR-KRAS double mutations in 3 samples.The mutation rate of EGFR was higher in females than that in males (39.5％ vs 29.4％,P=0.076),significantly increased in adenocarcinomas (38.7％) compared to that in the other forms of NSCLC (P＜0.01),but still lower than that reported in some Asian studies of advanced adenocarcinoma (-50％).Meanwhile,the mutation rate of KRAS was remarkably higher in males than that in females (16.6％ vs 9％,P=0.048),increased in adenocarcinomas compared to that in the other forms of NSCLC,but the difference was not significant (P=0.268).Samples harbored EGFR mutation were younger than those harbored KRAS mutation (P=0.031,5),and had significant difference in gender between the two groups (P＜0.01).Conclusion The mutation rate of EGFR in stag Ⅰ-Ⅲa NSCLC patients was lower than that in advanced NSCLC patients.And the percentage of the NSCLC patients with EGFRKRAS double mutations is 0.9％.
