Role of SOX4 on DDP Resistance in Non-small Cell Lung Cancer Cell of A549
10.3779/j.issn.1009-3419.2017.05.12
- VernacularTitle:SOX4对非小细胞肺癌细胞A549的顺铂耐药作用的影响
- Author:
LI WEI
1
;
LIU XU
;
ZHANG GUOQIAN
;
ZHANG LINLIN
Author Information
1. 天津中医药大学第一附属医院检验科
- Keywords:
Lung neoplasms;
A549;
SOX4;
Drug resistance;
Wnt pathway
- From:
Chinese Journal of Lung Cancer
2017;20(5):298-302
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Lung cancer is one of the most serious disease and the incidence of non-small cell lung cancer (NSCLC) is the highest in lung cancer. The main reason for the failure of chemotherapy is the tolerance to cisplatin. Transcriptional regulator SOX4 plays an important role in the occurrence and development of many tumors, and regulates Wnt signaling pathway by regulating the expression of β-catenin. We aimed to investigate the role of SOX4 on cisplat-in-resistance in NSCLC cell A549 cell. Methods The cisplatin-resistance lung cancer cell line A549/DDP was constructed by induction method in vitro, and cisplatin-resistance detected by CCK8 assay. Growth curves of A549 and A549/DDP was cal-culated. The expression level of SOX4 in A549 and A549/DDP cells were detected by Western blot. A549/DDP were knock-down of SOX4 by siRNA transfection, and the cisplatin-resistance of detected by CCK-8 assay, the expression level of β-catenin and Survivin were detected by real-time PCR and Western blot. Results The cisplatin-resistance cell line A549/DDP was constructed successfully, and its cisplatin-resistance is 13.7 times higher than in A549. There was no significance difference between A549 and A549/DDP in cell proliferation. The expression level of SOX4 is higher in A549/DDP than in A549. The cisplatin-resistance significantly decreased in A549/DDP cells after knockdown of SOX4 by siRNA transfection. The expres-sion level of β-catenin and Survivin significantly decreased in A549/DDP cells after knockdown of SOX4. Conclusion SOX4 can strengthen cisplatin-resistance of non-small cell lung cancer cell A549.
