Esketamine improves hypoxic-ischemic myocardial injury in neonatal rats by glycogen synthase kinase-3β/NOD-like receptor thermal protein domain-containing protein 3 pathway
- VernacularTitle:艾司氯胺酮通过糖原合成酶激酶-3β/NOD样受体热蛋白结构域蛋白3通路改善新生鼠缺氧缺血性心肌损伤的研究
- Author:
Xinyan LIU
1
;
Liyun DONG
;
Peipei ZHOU
;
Yongxue CHEN
;
Xinbo WANG
;
Yan SUN
Author Information
- Keywords: esketamine; myocardial injury; glycogen synthase kinase-3β; NOD-like recep-tor thermal protein domain-containing protein 3
- From: Journal of Clinical Medicine in Practice 2024;28(15):19-25
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effects of esketamine on hypoxic-ischemic myocardial injury in neonatal rats based on glycogen synthase kinase-3β/NOD-like receptor thermal protein do-main-containing protein 3(GSK-3 β/NLRP3)pathway.Methods Thirty neonatal rats were random-ly divided into sham operation group,model group and esketamine group,with 10 rats in each group.The rats in the sham operation group underwent a median incision in the neck to expose the bilateral common carotid arteries;the rats in the model group and the esketamine group underwent ligation of the common carotid arteries combined with a hypoxic environment to establish a model ofischemia and hypoxia;the rats in the esketamine group were given esketamine intervention(50 mg/kg).Left ven-tricular ejection fraction(LVEF),left ventricular fractional shortening(LVFS),left ventricular end-diastolic diameter(LVEDD),left ventricular end-systolic diameter(LVESD),serum creatine kinase isoenzyme(CK-MB),cardiac troponin Ⅰ(cTnⅠ),lactate dehydrogenase(LDH),tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-1β levels,myocardial injury,myocardial cell apopto-sis and apoptosis protein caspase 1/3/9 levels,neutrophil infiltration in myocardial tissue,and changes in GSK-3 β and NLRP3 protein levels in myocardial tissue were detected in each group.Results Compared with the sham operation group,the LVEF and LVFS were significantly de-creased and the LVEDD and LVESD were significantly increased in the model group,while the LVEF and LVFS were significantly higher and the LVEDD and LVESD were significantly lower in the esketamine group than in the model group(P＜0.05).The serum levels of CK-MB,cTnⅠ,LDH,TNF-α,IL-6,IL-1β,myocardial injury and apoptosis,caspase 1/3/9 protein levels in myo-cardial tissue sections,neutrophil counts,and GSK-3 β and NLRP3 protein levels were significantly increased in the model group,and these indicators were significantly lower in the esketamine group than in the model group(P＜0.05).Conclusion Esketamine improves hypoxic-ischemic myocar-dial injury in neonatal rats by inhibiting inflammation,and its mechanism of action is related to the GSK-3 β/NLRP3 pathway.
