Role of Autophagy in the Radiosensitivity of Human Lung Adenocarcinoma A549 Cells
10.3779/j.issn.1009-3419.2016.12.01
- VernacularTitle:自噬对人肺腺癌A549细胞放疗敏感性的影响
- Author:
XU LIYAO
1
;
WANG YONG
;
LIU QIN
;
LUO HUI
;
ZHONG XIAOJUN
;
LI YONG
Author Information
1. 南昌大学第一附属医院肿瘤科
- Keywords:
Autophagy;
Lung neoplasms;
DNA repair;
Radiosensitivity
- From:
Chinese Journal of Lung Cancer
2016;19(12):799-804
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Radiotherapy is an important treatment for lung cancer. The poor prognosis of lung cancer is largely caused by the high recurrence rate and metastasis of the tumor. Autophagy, which can be induced by radiotherapy, might be associated with DNA repair. The aim of this study is to investigate whether activating autophagy us-ing rapamycin can enhance the radiosensitivity of lung cancer cells and clarify the association of autophagy with DNA repair. Methods The human adenocarcinoma A549 cell line was selected as the experimental subject. The specimens were divided into three groups: control (N), radiation (R), and Rapamycin and radiation (R+ARPA). hTe protein levels of γ-H2AX, Rad51, Ku70/Ku80, p62, and LC3 were determined by Western blot. Autophagosome was observed under a transmission electron microscope, and SF was determined by colony formation assay.Results Compared with group R, the activity of autophagy and the protein expression levels of Rad51 and Ku70/80 were remarkably increased in group R+ARPA.ConclusionhTe radiosensitivity of lung cancer can be promoted by activating autophagy via treatment with Rapamycin, and the process may be associated with DNA repair.
