Effects of miR-424 on Proliferation and Migration Abilities in Non-small Cell Lung Cancer A549 Cells and Its Molecular Mechanism
10.3779/j.issn.1009-3419.2016.09.02
- VernacularTitle:miR-424对非小细胞肺癌A549细胞生长和侵袭的影响及分子机制
- Author:
LI HONGMIN
1
;
LAN HAITAO
;
ZHANG MING
;
AN NING
;
YU RUILIAN
;
HE YANGKE
;
GAN CHONGZHI
Author Information
1. 四川省医学科学院四川省人民医院肿瘤中心
- Keywords:
Lung neoplasms;
MiR-424;
Growth;
Invasion
- From:
Chinese Journal of Lung Cancer
2016;19(9):571-576
- CountryChina
- Language:Chinese
-
Abstract:
Background and objectivehTe inhibitory ability of miR-424 on the proliferation of renal carcinoma cell and the migration and invasion of cancer cells has been widely explored and demonstrated. However, the effects of miR-424 on non-small cell lung cancer (NSCLC) have not been systematically examined. In this study, detected the growth and inva-sion effect of miR-424 in NSCLC A549 cell. hTe migration and molecular mechanism of this cell are also detected.Methods NSCLC A549 cell was transfected with miR-424 and its inhibitor. Atfer transfection, the proliferation ability of A549 cell was detectedby CCK8 assay. hTen, the migration ability in A549 cell was detected by migration assays. Furthermore, the expression level of MMP2 and MMP9 in A549 was detected by Western blot and immune lfuorescence. hTe 3'UTR of E2F6 was cloned into luciferase reporter vector and its enzymatic activitywas detected to verify whether miR-424 can target E2F6. hTe expres-sion level of E2F6 in a549 cell atfer transfecing with miR-424 was detected by Western blot.Results Atfer transfection of miR-424, the proliferation and migration abilities were remarkably decreased and the expression level of MMP-2 and MMP-9 were down-regulated in A549. Moreover, MiR-424 inhibited the enzymatic activity of luviferase reporter vector of E2F6. Spe-ciifcally, the expression level of E2F6 was down-regulated in A549.ConclusionmiR-424 can inhibit the proliferation and migration abilities of A549 by negatively regulating the expression of E2F6.
