Investigation of Gene Expression Proifle of A549 Cells after Overexpression of GPC5 by High Throughput Transcriptome Sequencing
10.3779/j.issn.1009-3419.2016.08.11
- VernacularTitle:高通量转录组测序技术研究过表达GPC5对A549细胞基因表达影响
- Author:
ZHANG HAITIAN
1
;
WANG GUOXIANG
;
YANG XIN
;
QIU MANTANG
;
XU LIN
Author Information
1. 徐州医科大学临床医学系
- Keywords:
GPC5;
A549;
Proliferation;
Gene expression
- From:
Chinese Journal of Lung Cancer
2016;19(8):545-549
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Glypican-5 (GPC5) is an important tumor suppressor, while little is known about the impact of GPC5 on proliferation ability and gene expression in lung adenocarcinoma cell lines. Here, we stably over-expressed GPC5 in A549 cells and investigated the impact of cell proliferation ability and gene expression.MethodsA549 cells that stably overexpressed GPC5 were constructed by lentivirus. Cell counter kit 8 (CCK8), colony formation, EdU assay were conducted to analyze cell proliferation ability, and transcriptome sequencing was utilized to investigate gene expression proifle. ResultsCCK8 assay showed that compared with empty vector, overexpression of GPC5 signiifcantly inhibited cell pro-liferation rate in A549 cells and the number of colony was also decreased (181±17vs 278±23). EdU assay also conifrmed the percentage of positive staining cells decreased atfer GPC5 overexpression. Transcriptome sequencing revealed that 2,108 genes were differentially expressed atfer GPC5 overexpression. Among these differentially expressed genes, 47 genes of the Gene Ontology item “positive regulation of cell proliferation” were downregulated.ConclusionOverexpression of GPC5 inhibited proliferation ability in lung adenocarcinoma A549 cells and genes with the function of “positive regulation of cell proliferation” were downregulated.
