Performance and application of droplet digital polymerase chain reaction in rapid diagnosis of bloodstream infections
- VernacularTitle:微滴式数字聚合酶链式反应在血流感染快速诊断中的性能评价及应用
- Author:
Guiling LI
1
;
Chunmei LIU
;
Chuanli REN
Author Information
- Keywords: droplet digital polymerase chain reaction; bloodstream infection; blood culture; pathogen
- From: Journal of Clinical Medicine in Practice 2024;28(12):7-11,17
- CountryChina
- Language:Chinese
- Abstract: Objective To validate the performance of droplet digital polymerase chain reaction(ddPCR)reagents using clinical and standard strains,and to evaluate the effectiveness and practica-bility of ddPCR technology in clinical applications.Methods The concordance rate,specificity,pre-cision,and lower limit of detection of the ddPCR kit were validated using clinical and standard strains.Blood samples from 74 patients with suspected bloodstream infections were collected,and both ddPCR and blood culture methods were used to determine the pathogens in the patient's blood samples.Results The average detection time of ddPCR for pathogens of bloodstream infection was 3.5 hours,which was able to complete the detection of over a dozen common pathogens simultaneously.The con-cordance rate,specificity,precision,and lower limit of detection of the ddPCR kit for bloodstream in-fection pathogens all met clinical requirements.Among the 74 patients with suspected bloodstream in-fections,the positive detection rate using the ddPCR method was 64.86％,while was 40.54％using blood culture,with a statistically significant difference(P＜0.05).Conclusion The method of ddPCR can detect common pathogens of bloodstream infections rapidly,efficiently,and in large quantities,and its main detection performance can meet clinical needs.The combination of blood culture and ddPCR technology is conductive to early and rapid diagnosis of clinical bloodstream infection patients.
