Comprehensive Identification of MicroRNAs Regulated by Long Non-coding RNA MALAT1

VernacularTitle:系统性鉴定长非编码RNA MALAT1调控的微小RNA
Author: ZHANG HAITIAN ¹ ; WANG GUOXIANG ; YIN RONG ; QIU MANTANG ; XU LIN
Author Information

1. 徐州医科大学临床医学系
Keywords: Long non-coding RNA; MALAT1; MicroRNAs; Bioinformatics
From: Chinese Journal of Lung Cancer 2016;19(5):247-251
CountryChina
Language:Chinese
Abstract: Background and objective Long non-coding RNA (lncRNA) plays important regulatory roles in the development and invasion of various cancers. hTe aim of current study is to comprehensively identify microRNAs (miRNA) regulated by lncRNA MALAT1 via experimental and bioinformatics methods.Methods Antisense oligonucleotides (ASO) speciifcally targeting MALAT1 were designed and synthesized. Atfer knockdown of MALAT1 by ASO in A549 cells, miRNA expression changes were proifled by TqaMan Low Density Array (TLDA). Gene set enrichment analysis (GSEA) was used to search enriched miRNAs among differentially expressed genes atfer knockdown of MALAT1.ResultsAtfer effcient knock-down of MALAT1 by ASO, 153 miRNAs were differentially expressed, 131 up-regulated and 22 down-regulated. Among the 458 differentially expressed genes atfer MALAT1 silence, GSEA results revealed lots of enriched miRNAs. hTere were 28 over-lapped miRNAs between TLDA and GSEA results, suggesting these 28 miRNAs are regulated by MALAT1.Conclusion hTis study comprehensively identiifed MALAT1 regulated miRNAs, providing resources for further research.