Radiosensitization Induced by ANTP-SmacN7 Fusion Peptide in H460 Cell Line
10.3779/j.issn.1009-3419.2016.05.01
- VernacularTitle:ANTP-SmacN7融合肽对H460细胞的辐射增敏作用
- Author:
LIU BAONA
1
;
DU LIQING
;
XU CHANG
;
WANG YAN
;
WANG QIN
;
SONG ZHIYI
;
SUN XIAOHUI
;
WANG JINHAN
;
LIU QIANG
Author Information
1. 300192天津，中国医学科学院北京协和医学院放射医学研究所，天津市放射医学与分子核医学重点实验室
- Keywords:
Smac protein;
Tumor cell;
Apoptosis;
Radiosensitivity
- From:
Chinese Journal of Lung Cancer
2016;19(5):241-246
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective hTe curative effect of radiotherapy may be limited by the radioresistance of tumor. Mimetic compounds of Second mitochondria-derived activator of caspase (Smac) were hopeful to become new drugs of radiosensitization for tumor because they can increase radiation induced apoptosis in tumor cells. hTe aim of present study is to observe the radiosensitization effect of a new Smac mimetic ANTP-SmacN7 fusion peptide in H460 cell line.Methods In order to observe if the fusion peptide can enter into tumor cell, ANTP-SmacN7 fusion peptide was synthesized and linked by FITC. H460 cell was divided into control, radiation only, ANTP-SmacN7 only and ANTP-SmacN7 combined with radia-tion group. hTe cells were exposed by 0, 2, 4 and 6 Gy and the concentration of ANTP-SmacN7 was 20 μmol/L. Proliferation of H460 tumor cell was detected by WST-1 assay. hTere are four groups in the present study: control group, radiation group, ANTP-SmacN7 group and ANTP-SmacN7 combined with radiation group. Apoptosis was detected by lfow cytometry at 24 and 48 hours atfer the treatment of all the groups. hTe level of caspase3 and cleaved caspase3 were detected by Western blot as-say.Results ANTP-SmacN7 can enter into cells and promote the radiosensitization of H460 cell obviously (F=25.1,P<0.01, sensitivity enhancement ratio was 1.86). The treatment of ANTP-SmacN7 combined with radiation decreased the cloning forming effciency (χ2=45.2,P<0.01; χ2=40.3,P<0.01), activated caspase3 by promoting the expression of cleaved caspase3 and increased the apoptosis of H460 cell line.ConclusionANTP-SmacN7 fusion peptide had remarkably radiosensitization effect on H460 cell line. ANTP-SmacN7 fusion peptide might be hopeful to be applied in radiosensitization therapy as a new Smac mimetic polypeptide in the future.
