Nicotine Induced Lung Cancer Cells Epithelial-mesenchymal Transition and Promote Its Vitro Invasion Potential
10.3779/j.issn.1009-3419.2016.04.11
- VernacularTitle:尼古丁诱导肺癌细胞上皮间质转化促进其侵袭转移
- Author:
HOU YANXU
1
;
LI XUEBING
;
PAN ZHENHUA
;
ZU LINGLING
;
FAN YAGUANG
;
YOU JIACONG
;
WANG YULI
;
WANG MIN
;
CHEN PEIRUI
;
SHEN WANG
;
ZHOU QINGHUA
Author Information
1. 300052天津，天津市肺癌转移与肿瘤微环境重点实验室，天津市肺癌研究所，天津医科大学总医院
- Keywords:
Lung neoplasms;
Nicotine;
Epithelial-mesenchymal transition;
Invasion
- From:
Chinese Journal of Lung Cancer
2016;19(4):169-176
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Our previous study found that nicotine could induce lung cancer cell epithelial-mesenchymal transition (EMT). The aim of this study is to explore the relationship between nicotine-induced EMT and lung cancer invasion and metastasis. Methods Real-time PCR and Western blot were used to detect the expression changes of EMT-related markers, E-cadherin and Vimentin, in A549 lung cancer cells treated with nicotine;hTe transposition ofβ-catenin protein expression was determined by immunolfuorescence;Scratch test and Transwell invasion assay were used to detect the effects of nicotine on lung cancer cell migration and invasion. Results Nicotine can signiifcantly down-regulate the expressional level of E-cadherin mRNA and protein of A549 cells in a manner of dose and time-dependent (P<0.01, P<0.01);Nicotine can signiifcantly up-regulate the expressional level of Vimentin mRNA and protein of A549 cells in a manner of dose and time-dependent (P<0.01, P<0.01);Immunolfuorescence results showed thatβ-catenin protein was signiifcantly transfered to nucleus;Scratch test and Transwell assay showed that Nicotine could remarkably increase the migration and invasion poten-tial of lung cancer cells (P<0.01, P<0.01). Conclusion Nicotine can induce cancer cells EMT, and promote the invasion and metastasis ability of lung cancer cells.
