Inlfuence of Immunohistochemistry Scoring Criteria in Detecting EGFR Mutations
10.3779/j.issn.1009-3419.2015.12.05
- VernacularTitle:免疫组化染色评分对EGFR突变检测的影响
- Author:
FENG XIN
1
;
LIU CHANG
;
ZHONG DIANSHENG
;
XU DONGBO
;
NING CHAO
;
WANG JIE
Author Information
1. 天津医科大学总医院肿瘤内科
- Keywords:
Lung neoplasms;
Immunohistochemistry;
Epidermal growth factor receptor;
Mutation
- From:
Chinese Journal of Lung Cancer
2015;(12):740-744
- CountryChina
- Language:Chinese
-
Abstract:
Background and objectiveAppropriate immunohistochemistry (IHC) scoring criteria can guarantee the reliability of mutation detection results. Most current studies suggest that the “four-grade criterion” may be the best among all scoring systems. hTe aim of this study is to discuss the inlfuence of different four-grade immunohistochemistry scoring criteria on the test results.MethodshTree different four-grade immunohistochemistry scoring criteria were respectively used to evaluate the EGFR status of 83 cases of non-small cell of lung cancer (NSCLC) samples. hTe sensitivity, speciifcity, positive predictive value (PPV), negative predictive value (NPV), the agreement of each criterion compared with the gold standard, and whether statistical signiifcance exists between each criterion were calculated.Results No statistical difference was found among the three criteria in detectingEGFR mutations. All three criteria exhibit considerably better speciifcity than sensitivity. For samples with scores of “3+”, PPV could reach up to 100%.ConclusionNo deifnite best criterion is among different four-grade scoring criteria. Regardless of the kind of criterion used, the speciifcity of IHC method in detectingEGFR mutations is markedly better than the sensitivity. For samples with scores of “3+”, mutation status can be conifrmed, and samples can receive EGFR-TKI therapy directly.
