Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair.
10.4142/jvs.2011.12.3.295
- Author:
Kuldeep SINGH
1
;
Myrna M MILLER
;
Laura J KOHRT
;
Gail SCHERBA
;
Edgar F GARRETT
;
Richard L FREDRICKSON
Author Information
1. Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA. ksingh08@illinois.edu
- Publication Type:Brief Communication ; Evaluation Studies
- Keywords:
bovine viral diarrhea virus;
diagnostic tests;
hair;
persistent infection;
RT-PCR
- MeSH:
Animals;
Antibodies, Viral/analysis/diagnostic use;
Bovine Virus Diarrhea-Mucosal Disease/blood/*diagnosis/virology;
Cattle;
Diarrhea Virus 1, Bovine Viral/genetics/*isolation & purification;
Diarrhea Virus 2, Bovine Viral/genetics/*isolation & purification;
Hair/virology;
RNA, Viral/chemistry/genetics;
Real-Time Polymerase Chain Reaction/methods/*veterinary
- From:Journal of Veterinary Science
2011;12(3):295-297
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals were positive for the virus by qRT-PCR performed on the whole blood and when samples of more than 30 hairs were assayed. Additionally, qRT-PCR was performed on groups of 10 and 20 hairs harvested from 7 out of 23 immunohistochemical staining-positive calves. When groups of 20 and 10 hairs were tested, 6 and 4 animals, respectively, were positive for the virus.
