Investigational Study of Mesenchymal Stem Cells on Lung Cancer Cell Proliferation and Invasion
10.3779/j.issn.1009-3419.2015.11.03
- VernacularTitle:间充质干细胞对非小细胞肺癌细胞增殖和侵袭能力的初步探讨
- Author:
LI MEI
1
;
WU YI
;
LIU RENWANG
;
GUO LILI
;
XU TINGTING
;
CHEN JUN
;
XU SONG
Author Information
1. 天津医科大学总医院肺部肿瘤外科
- Keywords:
Lung neoplasms;
Mesenchymal stem cells;
Migration;
Proliferation
- From:
Chinese Journal of Lung Cancer
2015;(11):674-679
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Mesenchymal stem cells (MSC) are adult stem cells derived from meso-derm. Evidence has shown that MSC could migrate towards tumor tissue and differentiate into tumor associated ifbroblast in tumor microenvironment, which inlfuences tumor growth and metastasis. However, the reports of MSC in non-small cell lung cancer (NSCLC) are few and controversial. hTe aim of this study is to explore the chemotaxis of MSC towards NSCLC and to test the effects of MSC on the proliferation and invasion ability of NSCLC.MethodsTranswell assay was used to test MSC and NSCLC migration and invasion, and hTymidine incorporation assay was adopted to measure NSCLC cells proliferation. hTe expression of interleukin-6 (IL-6), insulinlike growth factor (IGF-1), vascular endothelial growth factor (VEGF) and dickkopf-related protein 1 (DKK1) of MSCs were determined by real time PCR. A549 lung cancer xenogratf animal tumor model was set up to evaluate the MSC effectin vivo.ResultsLung cancer cells could attract MSC tropism. MSC conditioned medium fa-vored lung cancer cell proliferation and lung cancer cells stimulated the expression of IL-6, IGF-1, VEGF and DKK1 on MSCs. In vivo animal study showed that the tumor with MSC injection grew much faster compared to control group.Conclusion MSCs could migrate towards NSCLC cells and favor tumor growth. In turn, NSCLC cells could stimulate the overexpression of cytokines on MSCs which are essential for the tumor growth.
