Application Progress of CRISPR/Cas9 System for Gene Editing in Tumor Research

VernacularTitle:CRISPR/Cas9基因编辑系统在肿瘤研究中的应用进展
Author: LIU CHAO ¹ ; LI ZHIWEI ; ZHANG YANQIAO
Author Information

1. 哈尔滨医科大学附属肿瘤医院内八科
Keywords: CRISPR/Cas9; Gene editing; Cancer genes
From: Chinese Journal of Lung Cancer 2015;(9):571-579
CountryChina
Language:Chinese
Abstract: TCRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-associated nuclease 9) gene editing system is a new type of gene editing technology developed based on the immune mechanism of ar-chaea resisting the invasion of exogenous nucleic acid. Compared with traditional gene editing system, CRISPR/Cas9 system is more effcient, easier operating, and less cytotoxic. Currently, CRISPR/Cas9 gene editing technology has been applied to many aspects of cancer research, including research on cancer genes, constructing animal tumor models, screening tumor resistance-associated and phenotypic-related genes and cancer gene therapy. In this review, the application of the CRISPR/Cas9 system in tumor research were introduced.