Effect of Long Non-coding RNA SPRY4-IT1 on Invasion and Migration of A549 Cells
10.3779/j.issn.1009-3419.2015.08.07
- VernacularTitle:人长链非编码RNA基因SPRY4-IT1对肺癌细胞A549侵袭和迁移能力的影响
- Author:
CHAI SONG
1
;
AN WENTING
;
GUO LILI
;
JIANG LE
;
GAO ZHISONG
;
LI SHUJUN
Author Information
1. 河北医科大学第二医院肿瘤中心
- Keywords:
Lung neoplasms;
Invasion;
Migration
- From:
Chinese Journal of Lung Cancer
2015;(8):487-492
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective hTe abnormal expression of human long chain non encoding RNA gene is related to many kinds of tumors. hTe aim of this study is to investigate the expression of long non-coding RNA maternally expressed gene 3 (SPRY4-IT1) in lung cancer (A549) cells, and to observe the effect of SPRY4-IT1 on the invasion and migra-tion of A549 cells.Methods hTe levels of SPRY4-IT1 in A549 was detected by Real-time PCR. hTe effects of SPRY4-IT1 on the invasion and migration of A549 cell were analyzed by MTT and Transwell assay. hTe expression of matrix metalloproteinase (MMP) family proteins was determined by Western blot.Results hTe invasion and migration of A549 cells were increased atfer SPRY4-IT1 over-expression. hTe cell spaces were narrower atfer SPRY4-IT1 over expression in the wound healing assay. Tran-swell assays showed that the numbers of transmembrane A549 cells were signiifcantly higher in SPRY4-IT1 over expression group than that in control group (P<0.05). Meanwhile, over expression of SPRY4-IT1 reduced the expression of MMP-2 and MMP-9.Conclusion Over expression of SPRY4-IT1 enhanced the invasion and migration of A549 cells. MMP-2 and MMP-9 might play an important role in this regulation.
