Study of the Changes on Tree Shrew Bronchial Epithelium Induced by Xuanwei Bituminous Coal Dust
10.3779/j.issn.1009-3419.2015.08.01
- VernacularTitle:宣威烟煤粉尘诱发树鼩支气管粘膜上皮改变的研究
- Author:
CHEN XIAOBO
1
;
HE MENG
;
LI GUANGJIAN
;
ZHOU YONGCHUN
;
ZHAO GUANGQIANG
;
LEI YUJIE
;
YANG KAIYUN
;
TIAN LINWEI
;
HUANG YUNCHAO
Author Information
1. 昆明医科大学第三附属医院 云南省肿瘤医院 胸外科一病区
- Keywords:
Tree shrews;
Xuanwei bituminous coal dust;
Bronchial epithelium;
Lung neoplasms
- From:
Chinese Journal of Lung Cancer
2015;(8):469-474
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Lung cancer is the type of cancer with the highest incidence and mortality in numerous countries and regions. Establishing an appropriate animal model that can be used to simulate lung cancer etiology, pathogenesis, and similar processes, is urgent. We explore the feasibility of establishing a lung cancer model induced by Xuan-wei bituminous coal dust PM10 (particulate matter with diameters of 10 μm or less), which affects bronchial epithelium of tree shrews.Methods hTe neck skin of adult tree shrews is dissected, and the thyroid cartilage is fully exposed. Subsequently, the weak part at the top of the thyroid cartilage is treated with intratracheal agents by perfusion via a special infusion needle punc-ture method. Regular X-ray examination and lung tissue biopsy were performed on the sacriifced animals to observe changes in pulmonary imaging and bronchial epithelial cells atfer perfusion of Xuanwei bituminous coal dust PM 10.ResultshTe tree shrews of the experimental group (exposed to bituminous coal dust) died in a week atfer perfusion with PM10, whereas no animal died until the end of the experiment in the blank control and the solvent control groups. Sections of lung tissue biopsy of the regularly killed tree shrews were stained with hematoxylin and eosin. hTe lung tissues of tree shrews in the experimental group showed a serial changes caused by bronchial epithelial hyperplasia, such as squamous metaplasia, dysplasia, and early invasive carcinoma, whereas no signiifcant pathological changes were observed in the blank control and solvent control groups. Conclusion Endotracheal infusion of Xuanwei bituminous coal dust PM10 induces lung cancer in tree shrews. hTus, the lung cancer model was established.
