MiR-373-3p Promotes Invasion and Metastasis of Lung Adenocarcinoma Cells
10.3779/j.issn.1009-3419.2015.07.07
- VernacularTitle:MiR-373-3p促进肺腺癌细胞的侵袭转移
- Author:
WU AIBING
1
;
LI JINMEI
;
WU KUNPENG
;
MO YANLI
;
LUO YIPING
;
YE HAIYIN
;
SHEN XIANG
;
LI SHUJUN
;
LIANG YAHAI
;
LIU MEILIAN
;
YANG ZHIXIONG
Author Information
1. 广东医学院附属医院肿瘤科
- Keywords:
Lung neoplasms;
MiR-373;
MMP-9;
MMP-14;
Invasion;
Metastasis
- From:
Chinese Journal of Lung Cancer
2015;(7):427-435
- CountryChina
- Language:Chinese
-
Abstract:
Background and objectiveLung cancer is the leading cause of cancer-related deaths worldwide, and metastasis is the major cause of death in lung cancer patients. MiR-373 is closely associated with invasion and metastasis in other tumor cells. hTis study explored the expression of miR-373-3p in non-small cell lung cancer (NSCLC) and its effect on the invasive and metastatic capabilities of lung adenocarcinoma cells, as well as their mechanisms of action.MethodshTe expression of miR-373-3p in NSCLC tissues and lung adenocarcinoma cell lines was detected by quantitative reverse transcrip-tion polymerase chain reaction. hTe roles of miR-373-3p in regulating lung adenocarcinoma cell invasion and metastatic prop-erties were analyzed with miR-373-3p mimic/inhibitor-transfected cells via Transwell chamber assay. Matrix metalloproteinase MMP-9 and MMP-14 protein levels were detected by Western blot in lung cancer cells atfer transfection.Results MiR-373-3p was upregulated in 51 NSCLC tissues and 5 NSCLC cell lines. Gain-of-function and loss-of-function studies showed that overexpression of miR-373-3p promoted H1299 cell migration and invasion, which resulted in upregulation of MMP-9 and MMP-14. By contrast, miR-373-3p knockdown inhibited these processes in A549 cells and downregulated the expression of MMP-9 and MMP-14.ConclusionOur results demonstrated that miR-373-3p participated in the invasion and metastasis of lung adenocarcinoma cells, partly by upregulation of MMP-9 and MMP-14.
