Screening of Highly Expressed CPEΔN Lung Cancer H1299 Cells
10.3779/j.issn.1009-3419.2015.06.03
- VernacularTitle:筛选高表达CPEΔN的H1299肺癌细胞株
- Author:
SUN JING
1
;
ZHANG GUIRONG
;
WANG HONGYUE
;
SHEN HUI
Author Information
1. 110042沈阳，辽宁省肿瘤医院生物治疗研究中心，辽宁省肿瘤研究所
- Keywords:
Lung neoplsms;
N-terminal truncated carboxypeptidase E;
Lentiviral vector
- From:
Chinese Journal of Lung Cancer
2015;(6):340-344
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective hTe N-terminal truncated carboxypeptidase E (CPEΔN) protein is a novel biomarker of tumor metastasis. hTis study screened the H1299 cell line with a highly expressed CPEΔN gene forin vivo imag-ing experiment.Methods Human CPEΔN gene was cloned into the luciferase lentiviral vector. H1299 cells transduced with CPEΔN or control lentiviral vectors were selected with 2 μg/mL puromycin. hTe expression of CPEΔN was identiifed through Western blot analysis, and luciferase activity was measured using luciferase reporters.Results hTe human CPEΔN lentiviral expression vector was successfully constructed. hTe transfection rate of H1299 cells by the lentivirus achieved 80%, with an infection multiplicity of 20. hTe H1299 cell line with high CPEΔN (H1299-CPEΔN) expression was established, with an in-crease in CPEΔN expression by four times compared with the control lentivirus-transfected H1299 cell line (H1299-control). As H1299-CPEΔN and H1299-control can effectively decompose luciferase substrates, they can be applied in in vivo imaging. Conclusion H1299-CPEΔN and H1299-control can be used inin vivo imaging experiment for further research on molecular mechanisms and signal transduction to elucidate the role of CPEΔN in lung cancer metastasis.
