Immunohistochemical detections ofEGFRstatus in NSCLC
10.3779/j.issn.1009-3419.2015.04.01
- VernacularTitle:免疫组化法检测NSCLC患者EGFR突变的相关研究
- Author:
WANG JIE
1
;
LIU CHANG
;
ZHONG DIANSHENG
;
XU DONGBO
;
NING CHAO
;
MA QING
Author Information
1. 天津医科大学总医院肿瘤科
- Keywords:
Lung neoplasms;
Epidermal growth factor receptor;
Mutation;
Immunohistochemistry;
Liquid chip technology
- From:
Chinese Journal of Lung Cancer
2015;18(4):212-218
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Patients with non-small cell lung cancer (NSCLC) harboring mutations of the epidermal growth factor receptor (EGFR) respond well to EGFR-tyrosine kinase inhibitor therapy. Immunohistochemistry (IHC) is a simple and widely used technique in clinical pathology laboratories. IHC also features cost effectiveness and rapid detection ofEGFR mutations compared with molecular methods. hTis study aims to determine the accuracy of IHC forEGFR mutation detection in NSCLC.Methods Specimens (obtained from surgery or biopsy) from 97 NSCLC cases were stained through IHC with mutation-specific antibodies. The clinicopathological features of patients with positive immunostaining results were analyzed. Positive specimens were subjected to liquid chip technology to detect the actual EGFR status. Forty NSCLC specimens obtained from surgery and conifrmed to haveEGFR mutations through liquid chip technology were collect-ed. These specimens were then subjected to IHC analyses with mutation-speciifc antibodies. The sensitivity of IHC in detect-ingEGFR mutations was calculated.Results Seventeen of the 97 NSCLC specimens were stained positive, and positive results were mostly observed in females, patients with adenocarcinoma, and non-smokers. About 76.9% of specimens with positive IHC results harbored mutations. The sensitivity of IHC was 40% among the 40 cases identiifed as containingEGFR mutations through liquid chip technology.Conclusion The strong positive immunostaining result is accurate, but the sensitivity of the method may not be optimal and signiifcantly varies in different studies. The widespread application of IHC in clinics must be further investigated.
