Clinical Research of EGFR and KRAS Mutation in Fine Needle Aspiration Cytology Specimens of Non-small Cell Lung Carcinoma
10.3779/j.issn.1009-3419.2015.04.05
- VernacularTitle:细针吸取细胞学标本检测非小细胞肺癌EGFR、KRAS突变的探讨
- Author:
ZHANG ZHIHUI
1
;
WU XILAN
;
YING JIANMING
;
LI JUNLING
;
QIU TIAN
;
GUO HUIQIN
;
ZHAO HUAN
;
SHAN LING
;
LING YUN
Author Information
1. 北京中国医学科学院肿瘤医院病理科
- Keywords:
Lung neoplasms;
Epidermal growth factor receptor;
Fine needle aspiration cytology;
Gene
- From:
Chinese Journal of Lung Cancer
2015;18(4):199-205
- CountryChina
- Language:Chinese
-
Abstract:
Background and objectiveEpidermal growth factor receptor (EGFR) and KARS must be detected mu-tation status before patients of lung cancer use targeted drugs. The aim of this study is to elucidate the signiifcance ofEGFR and KARS mutation in ifne needle aspiration (FNA) cytology suspension specimens of non-small cell lung carcinoma.Methods EGFR gene exons 18-21 andKARS codons 12, 13 of exons 2 were performed by Real-time PCR methods in ifne needle aspira-tion cytology suspension specimens of lymph nodes.Results 85 metastasis lymph nodes were detected in ifne needle aspira-tion cytology samples of lung cancer.EGFR mutation rate was 37.3%.KARS mutation rate was 7.2%. 19 formalin ifxed paraffn-embedded tissue specimens were available and match cytology specimens. Analysis ofEGFRmutation status in those samples revealed agreement with the results obtained in cytological samples (kappa=1.0). Clinical follow-up was available for 13 who presented with stage IV disease. Based on the identiifcation of such mutations, these patients received subsequent therapy with a TKI in clinic. We observed two cases complete remission (16.7%) and 8 cases partial remission (66.7%) and three had ongo-ing stable disease.ConclusionFine-needle aspiration cytology samples were detectedEGFR andKARS mutation. The meth-od which collects samples was easier, simple and convenient. This method has higher application value in clinical treatment.
