EFEMP1 Suppresses Growth and Invasion of Lung Cancer Cells by Downregulating Matrix Metalloproteinase-7 Expression
10.3779/j.issn.1009-3419.2015.02.08
- VernacularTitle:EFEMP1通过下调MMP-7表达抑制肺癌细胞生长和侵袭
- Author:
LANG YUANYUAN
1
;
MENG JIE
;
SONG XIAOMENG
;
CHEN XIAOJUN
Author Information
1. 天津儿童医院检验科
- Keywords:
EFEMP1;
MMP-7;
Lung neoplasms;
Invasion
- From:
Chinese Journal of Lung Cancer
2015;(2):92-97
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective EFEMP1, a member of ifbulin family proteins, is a very important extracel-lular matrix protein which is involved in cell metabolism and its role in tumor occurrence and progression is still poorly under-stood. hTe aim of this study is to investigate the functional effect and mechanism of EFEMP1 in lung cancer cell growth and invasion. Methods EFEMP1 expression in lung cancer cells was determined by Western blot. hTe promoter methylation status of EFEMP1 was detected by methylation-speciifc PCR (MSP). Atfer transfection of control or EFEMP1 vector in lung cancer cells, the ability of colony formation and invasion was detected by colony formation experiment and matrigel invasion method. Western blot and real-time PCR were used to detect matrix metalloproteinase-7 (MMP-7) expression. Luciferase assay was used to detect expression of MMP-7 reporter construct transfected with or without EFEMP1 in lung cancer cells. Results Western blot result showed EFEMP1 expression was downregulated in lung cancer cells. hTe promoter region of EFEMP1 was methyl-ated in A549 and H1299 and atfer treatment with 5-aza-2’-deoxycytidine, the EFEMP1 expression was upregulated. hTe growth and invasion of A549 and H1299 were all signiifcantly suppressed by transfecting with EFEMP1 and the MMP-7 expression was dowanregulated by EFEMP1 as well. Expression activity of MMP-7 reporter construct was decreased by cotransfecting with EFEMP1. Conclusion Collectively, these results suggest that EFEMP1 functions as a suppressor of lung cancer growth and inva-sion. Epigenetic silencing of EFEMP1 promotes lung cancer invasion and metastasis by activating MMP-7 expression.
