Analysis of EML4-ALK Gene Fusion Mutation in Patients with Non-small Cell Lung Cancer
10.3779/j.issn.1009-3419.2015.02.05
- VernacularTitle:非小细胞肺癌患者EML4-ALK融合基因突变研究
- Author:
WANG XUZHOU
1
;
CHEN WEISHENG
;
YU YINGHAO
Author Information
1. 南京军区福州总医院病理科
- Keywords:
Lung neoplasms;
EML4-ALK fusion gene;
Immunohistochemistry;
ARMS;
Fluorescence in situ hybrid-ization
- From:
Chinese Journal of Lung Cancer
2015;(2):80-84
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Non-small cell lung cancer (NSCLC) is the main type of lung cancer, and the related locus mutation detection research has become a hot direction of molecular targeted therapy, studying on gene mu-tation status of echinodem microtubule associated protein like 4-Anaplastic lymphoma kinase (EML4-ALK) and epidermal growth factor receptor (EGFR), detecting the sensitivity of EML4-ALK gene fusion and gene mutation of EGFR. Methods EML4-ALK gene fusion in 85 cases of paraffn embedded tumor tissue and adjacent lung tissue was detected with the applica-tion of immunohistochemistry (IHC), Scorpions ampliifcation refractory mutation system (Scorpions ARMS) lfuorescence quantitative PCR and lfuorescence in situ hybridization (FISH) technology, and EGFR gene in 18, 19, 20 and 21 exon mutation status was detected with the application of ARMS method. Results In 115 cases of NSCLC, IHC showed 32 cases with ALK (D5F3) expression, the expression rate was 27.8%;ARMS showed 27 cases with EML4-ALK fusion gene mutation, the muta-tion detection rate was 23.5%;53 cases were detected with EGFR mutation, the mutation rate was 46%. While FISH showed 23 cases with EML4-ALK fusion gene mutation, the detection rate was 20%, slightly lower than the ARMS detection results, suggesting that ARMS more sensitive. Conclusion hTe application of IHC, ARMS lfuorescence quantitative PCR and FISH technology can make a rapid and accurate evaluation of EML4-ALK gene fusion.
