Assessment of ALK Rearrangement in Non-small Cell Lung Cancer：Using Enhancing Immunohistochemical Way and Fluorescence in situ Hybridization

VernacularTitle:增强免疫组化和原位杂交方法检测非小细胞肺癌的ALK重排的临床可行性
Author: MENG HUI ¹ ; GAO XIANZHENG ; ZHANG LAN ; LIU FANG ; LI WENCAI
Author Information

1. 郑州大学第一附属医院病理科
Keywords: Lung neoplasms; ALK; Ventana-IHC; FISH
From: Chinese Journal of Lung Cancer 2015;(2):75-79
CountryChina
Language:Chinese
Abstract: Background and objective Besides epidermal growth factor receptor (EGFR) mutation, the non-small cell lung cancer (NSCLC) of anaplastic lymphoma kinase (ALK) rearrangement becomes another important clinical subtype. A speciifc and high-sensitive and economical detection way is convenience for identiifcation of ALK positive NSCLC quickly and accurately. So the objective of our research is to detect ALK rearrangement in 172 cases of NSCLC by using enhancing immunohistochemical way (ventana-IHC, V-IHC). Methods ALK rearrangement in 172 NSCLC samples was detected by us-ing V-IHC, and positive staining cases were further veriifed by lfuorescence in situ hybridization (FISH). Results Among 172 NSCLC cases, there were 12 positive staining. hTe positive results were conifrmed by FISH and 11 cases were FISH positive. hTe overall concordance between V-IHC and FISH is 91.7%(11/12). Conclusion hTe V-IHC method is a reliable method for ALK arrangement and could be used in clinical screen and diagnosis.