Signifcance of Detection of Serum Carbonic Anhydrase IX in the Diagnosis of Lung Cancer
10.3779/j.issn.1009-3419.2015.01.05
- VernacularTitle:血清碳酸酐酶IX检测对肺癌的诊断意义
- Author:
CHENG FANGYUAN
1
;
WANG XIAOE
;
ZHONG DIANSHENG
;
SUN LINLIN
;
WANG QIAN
;
LIU CHANG
Author Information
1. 天津医科大学总医院肿瘤科
- Keywords:
Lung neoplasms;
Carbonic anhydrase IX;
Diagnosis
- From:
Chinese Journal of Lung Cancer
2015;(1):29-33
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Carbonic anhydrase IX (CAIX) is a transmembrane protein involved in the metabolism of tumor cells. CAIX is expressed in only a few normal tissues but is overexpressed in various tumor types. Te aim of this study is to detect the serum CAIX level of patients with lung cancer, evaluate the significance of CAIX detection in the diagnosis of lung cancer, and analyze the serum CAIX level among diferent pathological types and TNM stages of lung can-cer. Methods Forty-seven patients with lung cancer and 31 healthy subjects were selected to participate in this study. Serum CAIX level was examined through enzyme-linked immunosorbent assay (ELISA). Te subjects were grouped according to histological type and TNM staging, and serum CAIX level was compared among the groups. With pathological diagnosis as the gold standard, receiver operating characteristic curve of the serum CAIX level was established for the diagnosis of lung cancer. Results Te CAIX serum level was significantly higher in patients with lung cancer than that in the healthy group (P<0.001). Te serum CAIX level in patients with squamous cell carcinoma and small cell carcinoma was also significantly higher than that in patients with adenocarcinoma. No statistically significant diferences were observed in the serum CAIX level between I+II and III+IV staging. Te AUC of serum CAIX level was 0.961. At a threshold level of 115.115 pg/mL, sensitivity and specificity were 95.7% and 90.3%, respectively. Conclusion Detection of the serum CAIX level through ELISA exhibits high sensitivity and specificity and is important for the diagnosis of lung cancer.
