99mTc-octreotide Receptor Scintigraphy in NCI-H446 Small Cell Lung Cancer Nude Mice Model
10.3779/j.issn.1009-3419.2015.01.01
- VernacularTitle:人小细胞肺癌NCI-H446裸鼠模型的99mTc-octreotide受体显像研究
- Author:
LI CHAO
1
;
ZUO SHUYAO
;
WANG XUFU
;
LIU XINFENG
;
WANG GUOMING
;
WU FENGYU
Author Information
1. 青岛大学医学院附属医院核医学科
- Keywords:
Technetium;
Octreotide;
Small cell lung cancer;
Nude mouse;
Receptor imaging
- From:
Chinese Journal of Lung Cancer
2015;(1):1-7
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective For highly aggressive small cell lung cancer (SCLC), early diagnosis is im-portant for its prognosis, but the current inspection methods are more limited, with poor specificity of the traditional imaging methods, and the high cost of PET/CT, difcult to popularization and application. SCLC is kind of neuroendocrine tumors, high expression of somatostatin receptors, which is the cornerstone of its early molecular imaging diagnosis. Te aim of this study is to observe the biodistribution and metabolism of 99mTc-octreotide in normal and the human SCLC bearing nude mice. Methods Dynamic and static scintigraphy at 0.5 h, 2 h, 3 h, 4 h were performed in both normal and tumor bearing nude mice afer intravenous injection of 99mTc-octreotide. Te technique of drawing region of interest (ROI) was used to obtain the aver-aged pixel counts and the activity-time (A-T) curve of brain, heart, lung, liver, kidney, tumor, respectively. Results ① Te biodistribution study in normal nude mice showed highest uptake in kidney and liver, lower in lung and heart, lowest in brain. Most 99mTc-octreotide was excreted via kidney. ② All tumors were displayed clearly at 3 h postinjection of 99mTc-octreotide. Te averaged T/N ratio at 0.5 h, 2 h, 3 h, 4 h postinjection of 99mTc-octreotide was 1.163±0.03, 2.08±0.12, 3.03±0.23, 2.689±0.31, respectively (F=51.69, P<0.000,1). Te radioactivity of tumor was lower than liver, and similar with the lung. Te curve of tu-mor showed a radioactivity peak at 2 min-3 min postinjection. Conclusion 99mTc-octreotide receptor imaging on nude mice bearing SCLC shares high positive rate, especially at 3 h postinjection.
