Inhibitory Effect of Endostar on Lymphangiogenesis in Non-small Cell Lung Cancer and Its Effect on Circulating Tumor Cells
10.3779/j.issn.1009-3419.2014.10.03
- VernacularTitle:重组人血管内皮抑素对非小细胞肺癌淋巴管生成的抑制及对循环肿瘤细胞的影响
- Author:
SHANG LIQUN
1
;
ZHAO JIE
;
WANG WEI
;
XIAO WANG
;
LI JUN
;
LI XUECHANG
;
SONG WEIAN
;
LIU JUNQIANG
;
WEN FENG
;
YUE CAIYING
Author Information
1. 海军总医院胸外科
- Keywords:
Recombinant human endostatin injection;
Tumor-bearing model;
Microlymphatic vessel density;
Posi-tive expression rate;
Circulating tumor cells
- From:
Chinese Journal of Lung Cancer
2014;(10):722-729
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective It is unclear how could endostatin effect tumor lymphangiogenesis? hTe aim of this study is to explore inhibitory effect of recombinant human endostatin injection (endostar) on lymphangiogenesis in non-small cell lung cancer (NSCLC) tissue and its effect on circulating tumor cells (CTC) in peripheral blood. Methods Tu-mor-bearing model nude mice were divided into eight groups randomly (n=7), including control group, cisplatin group, several concentration endostar groups and endostar plus cisplatin groups. Continuous administration of Endostar for two weeks, ob-served one week atfer the end of administration. Using HE staining and immunohistochemical staining to diagnose the tumor tissue and suspect metastasis lymph nodes, detected vascular endothelial growth factor (VEGF)-C, VEGF-D, VEGF receptor (VEGFR)-3 expression level and microlymphatic vessel density (MLVD) of tumor tissue. Enrichment of circulating tumor cells in peripheral blood used immunomagnetic negative selection strategy, used immunolfuorescence staining to diagnose and count CTCs. Results Microlymphatic vessel density and the positive expression rate of VEGF-C, VEGF-D, VEGFR-3 in three endostar groups and three endostar plus cisplatin groups were signiifcantly less than those in control group and cisplatin group. Microlymphatic vessel density and the positive expression rate of VEGF-C, VEGF-D, VEGFR-3 in endostar plus cisplatin group and endostar group with high endostar concentration were signiifcantly less than those with low endostar concentration;hTere was a signiifcant positive correlation between microlymphatic vessel density and the positive expression rate of VEGF-C, VEGF-D, VEGFR-3. hTe number of circulating tumor cells in endostar plus cisplatin groups were signiifcantly less than that of endostar or cisplatin alone. Conclusion Endostar could inhibit tumor lymphangiogenesis and reduce tumor cells into the bloodstream through the lymphatic. Inhibitory effect concerned with drug concentrationwith a dose-dependant.
