MiR-192 Confers Cisplatin Resistance by Targeting Bim in Lung Cancer
10.3779/j.issn.1009-3419.2014.05.04
- VernacularTitle:MiR-192靶向负调控Bim表达诱导肺癌顺铂耐药
- Author:
ZHANG FANG
1
;
LI YANG
;
WU HENG
;
QI KANG
;
YOU JIACONG
;
LI XUEBING
;
ZU LINGLING
;
PAN ZHENHUA
;
WANG YULI
;
LI YONGWEN
;
LI YING
;
WANG MIN
;
SHEN WANG
;
ZHOU QINGHUA
Author Information
1. 300052 天津，天津市肺癌转移与肿瘤微环境重点实验室，天津市肺癌研究所，天津医科大学总医院
- Keywords:
Lung neoplasms;
miRNA microarray;
RT-PCR;
miR-192;
Bim
- From:
Chinese Journal of Lung Cancer
2014;(5):384-390
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Cisplatin is the ifrst-line drug for the chemotherapy of non-small cell lung cancer (NSCLC), but the acquired chemoresistance restricted the effect of its treatment. hTe aim of this study is to validate the miRNAs related to the Cisplatin resistance in lung cancer and elucidate the molecular mechanisms. Methods We performed miRNA microarray and RT-PCR to obtain the aberrant differential expressed miRNAs between A549 and its paired Cisplatin-resistant cell line A549/DDP cells, and then we investigated the biological functions of miR-192, which is the aberrant differen-tial expressed miRNA. Atfer transfection of the miR-192 into A549 cells, we measured the half inhibition concentration (IC50), cell apoptosis of the trasfectant cells, and then we used biological sotfwares and dual-luciferase report assay to explore the target gene of the miR-192, which was further validated by RT-PCR and Western blot. Result MiR-192 was highly over-expressed in A549/DDP cells , whose quantity was 37.59±0.35 fold higher than that in A549 cells. Overexpression of miR-192 in A549 cells signiifcantly conferred resistance to Cisplatin and inhibited apoptosis. By contrast, down-expression of miR-192 in A549/DDP cells remarkably restrained the Cisplatin resistance and induced apoptosis. MiR-192 binded to Bim 3’-UTR and negatively regulated Bim expression at the post-transcriptional level in lung adenocarcinoma cells. Conclusion Our data suggested that miR-192 induced Cisplatin-resistance and inhibited cell apoptosis in lung cancer via negative targeting Bim expression.
