Thymosin beta 10 Prompted the VEGF-C Expression in Lung Cancer Cell
10.3779/j.issn.1009-3419.2014.05.03
- VernacularTitle:胸腺素β10在肺癌细胞中促进VEGF-C表达机制研究
- Author:
LI ZIXUAN
1
,
2
;
QU LIANYUE
;
ZHONG HONGSHAN
;
XU KE
;
QIU XUESHAN
Author Information
1. 110001沈阳，中国医科大学附属第一医院病理科，基础医学院病理学教研室
2. 中国医科大学附属第一医院放射科，辽宁省影像诊断与介入治疗重点实验室
- Keywords:
Lung neoplasms;
hTymosinβ10;
Phosphorylated protein kinase B;
Vascular endothelial growth factor C
- From:
Chinese Journal of Lung Cancer
2014;(5):378-383
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Our previous study found that thymosinβ10 overexpressed in lung cancer and positively correlated with differentiation, lymph node metastasis and stage of lung cancer. In this reasearch we aim to study the effects and mechanism of exogenous human recombinant Tβ10 on the expression of VEGF-C on non-small cell lung can-cer. Methods Atfer SPC, A549 and LK2 cells were treated with 100 ng/mL recombinant human Tβ10, the mRNA level of VEGF-C were detected by RT-PCR. hTe mean while the protein expression of VEGF-C, P-AKT and AKT were determined by Western blot assay. Results Exogenous recombinant human Tβ10 were signiifcantly promote the expression levels of VEGF-C mRNA and protein while promoting the phosphorylation of AKT. Exogenous Tβ10 can promote the expression of VEGF-C mRNA and protein in lung cancer cell lines A549 and LK2 (P<0.05), and this effect can be inhibited by use AKT inhibitor LY294002 (P<0.05). Conclusion Tβ10 human recombinant proteins can promote the expression of VEGF-C by activating AKT phosphorylation in lung cancer cell lines.
