Signiifcance of Epithelial-mesenchaymal Transition Phenotype in Invasive Tumor Front Cells of Lung Squamous Cell Carcinoma
10.3779/j.issn.1009-3419.2014.04.05
- VernacularTitle:肺鳞癌肿瘤浸润前沿细胞的EMT表型特点及临床意义
- Author:
SONG YINGHUA
1
;
ZHANG CAIQING
;
CAO ZHIXIN
;
XU JIAWEN
;
WANG LINGCHENG
;
LIN XIAOYAN
Author Information
1. 山东大学附属山东省千佛山医院呼吸内科
- Keywords:
Lung squamous cell carcinoma;
Invasive tumor front;
E-cadherin;
β-catenin;
Vimentin
- From:
Chinese Journal of Lung Cancer
2014;(4):315-320
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective hTe invasive tumor front (ITF) refers to cells or invasive nests in the junc-tional region of a tumor and its host. hTe ITF contains the most invasive cells of a tumor, and has a high prognostic value in carcinoma. hTe aim of this study is to investigate the epithelial-mesenchymal transformation phenotype in ITF cells of lung squamous cell carcinoma (SCC), and analyze the relationship between clinicopathological features and clinical outcomes of pa-tients. Methods Semiquantitative immunohistochemistry was used to examine the expression of epithelial markers (E-cadherin andβ-catenin) and mesenchymal marker (vimentin) in 104 lung SCC tumor tissues. Results A decrease in E-cadherin expres-sion in ITF cells was observed in 56 of 104 (53.8%) tumors from patients. hTis result was markedly lower than that of non-ITF cells, which eventually developed metastatic tumors and were also associated with death (P=0.04). Vimentin expression was observed in 44 of 104 (42.3%) ITF cells, which was much higher than that of non-ITF cells. hTe downregulation of E-cadherin and overexpression of vimentin were associated with tumor invasive pattern, lymphatic metastasis, and poor prognosis (P<0.01). hTe expression ofβ-catenin was 67.3%(70/104) in ITF cells. Moreover, ITF cells showed more nuclear and plasma-positive cells, which were closely associated with metastasis (P<0.01). Conclusion hTe loss in expression of E-cadherin/β-catenin and overexpression of vimentin in ITF cells may be associated with poor prognosis of lung SCC patients.
