Effects of Tumor Suppressor Gene TCF21 on the Proliferation, Migration and Apoptosis of A549 Cells
10.3779/j.issn.1009-3419.2014.04.03
- VernacularTitle:抑癌基因TCF21对肺癌细胞A549增殖、凋亡和迁移的影响
- Author:
HU SONG
1
;
YANG NUO
;
CHEN MINGWU
;
GUO JIANJI
;
XIAN LEI
Author Information
1. 广西医科大学第一附属医院心胸外科
- Keywords:
A549;
Tumor suppressor;
Transcription factor 21;
Apoptosis;
Cell proliferation
- From:
Chinese Journal of Lung Cancer
2014;(4):302-307
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective TCF21, a newly discovered gene, exhibits tumor suppressor function in a variety of tumors. hTis study aims to observe the effects of TCF21 on the proliferation, apoptosis and migration of A549 human lung adenocarcinoma epithelial cells. Methods TCF21 was overexpressed in A549 cells via lentiviral transfection. Fluorescence-based quantitative polymerase chain reaction and Western blot analysis were used to analyze the expression of the target gene. Transwell, proliferation assay, and lfow cytometry were applied to detect the effect of TCF21 overexpression on the migration, proliferation, and apoptosis of A549 cells atfer transfection. Results hTe proliferation and migration of A549 cells were inhib-ited, and the apoptotic rate was increased by overexpressing TCF21. Conclusion hTe tumor suppressor gene, TCF21, signiif-cantly inhibits the proliferation and migration, as well as facilitates early apoptosis of A549 cells.
