High Resolution Melting Curve Method for Detection of BIM Deletion
10.3779/j.issn.1009-3419.2014.03.10
- VernacularTitle:高分辨率熔解曲线法在BIM基因2,903 bp片段插入/缺失检测中的应用
- Author:
XIA JINJING
1
;
BAI HAO
;
XIONG LIWEN
;
LI RONG
;
YAN BO
;
SHAO MINHUA
;
HAN BAOHUI
Author Information
1. 上海交通大学医学院附属上海市胸科医院呼吸内科
- Keywords:
High resolution melting curve method;
BIM gene;
Lung neoplasms
- From:
Chinese Journal of Lung Cancer
2014;(3):238-242
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective hTe aim of this study is to establish a HRM (high resolution melting curve) method for detection of deletion in human BIM gene and to detect this site deletion with the above method in 30 lung cancer samples and 30 normal samples. Methods The primers for detection of BIM deletion were designed and synthesized. The HRM method for geng deletion was established. And select the part of samples to detect BIM delection by normal PCR and sequencing assay. hTe Tm value of wild type PCR products was higher than that of the deletion PCR products. hTe difference of the corresponding Tm value is 2.5 oC. Results By detection with HRM methods, 1 samples were conifrmed to be mutant, 7 samples were conifrmed to be heterozygous and the other 22 samples were all wild type in the lung cancer samples. 2 samples were conifrmed to be heterozygous and the other 28 samples were all wild type in the normal samples. Conclusion hTe HRM method for detection of BIM deletion established in this study is a sensitive, accurate, simple and high throughput method.
