Expression of MiRNA-221 in Non-small Cell Lung Cancer Tissues and Correlation with Prognosis
10.3779/j.issn.1009-3419.2014.03.07
- VernacularTitle:非小细胞肺癌组织miRNA-221表达及其与预后的关系研究
- Author:
LV JUNJIE
1
,
2
;
XU LEI
;
XU YOUTAO
;
QIU MANTANG
;
YANG XIN
;
WANG JIE
;
YIN RONG
;
XU LIN
Author Information
1. 210009 南京，南京医科大学附属江苏省肿瘤医院胸外科，江苏省恶性肿瘤分子生物学及转化医学重点实验室
2. 223300 淮安，淮安市第一人民医院胸外科
- Keywords:
miRNA-221;
Lung neoplasms;
Gene expression;
Prognosis
- From:
Chinese Journal of Lung Cancer
2014;(3):221-225
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective MicroRNAs were important regulators of tumors and the expression of miRNA-221 was associated with malignant proliferation and invasion in many tumors. hTe aim of this study is to explore the expression of miRNA-221 in non-small cell lung cancer (NSCLC) tissues and its correlation with prognosis. Methods We ret-rospectively analyzed the clinical characteristics of 117 NSCLC patients who underwent surgery in our hospital from Novem-ber 2005 to January 2007. Real-time PCR was used to detect the expression of miRNA-221. Chi-square test was utilized to ana-lyze the relationship between miRNA-221 expression and clinicopathologic features. Survival curves were plotted by using the Kaplan-Meier method and compared by the Log-rank test. A Cox proportional hazard regression model was applied to examine the joint effect of covariants. A P-value less than 0.05 was evaluated as statistically signiifcant. Results hTe patients were di-vided into two groups according to the relatively expression of miRNA-221. No statistically signiifcance was observed between the expression of miRNA-221 and the clinicopathologic parameters, including gender (χ2=0.070, P=0.791), histology (χ2=0.414, P=0.520), p-TNM stage (χ2=0.068, P=0.794) and history of smoking (χ2=0.206, P=0.650). Kaplan-Meier analysis showed that high expression of miRNA-221 was closely associated with a shorter survival time (P<0.001). Finally, both univariate and multivariate analyses demonstrated that high miRNA-221 expression might be a poor prognostic marker of NSCLC patients. Conclusion Our results indicated that down-regulation of miRNA-221 was associated with poor prognosis of patients with NSCLC. MiRNA-221 may serve as a molecular prognosis marker for patients with NSCLC.
