Radio-labeling of T7 Peptide with 99mTc and Its Biodistribution in Nude Mice Bearing Non-small Cell Lung Cancer
10.3779/j.issn.1009-3419.2014.03.02
- VernacularTitle:99mTc标记T7肽及其在裸鼠非小细胞肺癌模型体内的生物分布研究
- Author:
HAO YUMEI
1
;
HE XIN
;
ZHOU XIAOLIANG
;
MENG AIMIN
;
LIU JIANFENG
;
LIU JINJIAN
;
SONG NALING
Author Information
1. 300192 天津，中国医学科学院北京协和医学院放射医学研究所，天津市放射医学与分子核医学重点实验室
- Keywords:
99mTc-T7;
Tumstatin T7 peptide;
Integrinαvβ3;
Lung cancer imaging;
Biodistribution
- From:
Chinese Journal of Lung Cancer
2014;(3):189-196
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Lung cancer is a malignant tumor with high mortality rates. hTis study aims to develop potential candidates of integrinαvβ3 imaging agents, which can facilitate the diagnosis and treatment of lung cancer. Methods hTe T7 peptide was labeled with carbonyl technetium. hTe thin layer chromatography with acetone as the development system was performed to investigate the purity and stability of 99mTc-T7. hTe binding affnity of 99mTc-T7 with NCI-H157 tumor cells was determined. hTe biodistribution of 99mTc-T7 in nude mice bearing non-small cell lung carci-noma was observed atfer injection of 99mTc-T7 at 0.5 h, 1 h, 2 h, 4 h, and 8 h, and the radioactive ratio of tumor (T) and non-tumor tissues (NT) was calculated. Results 99mTc labeled T7 had high radiochemical purity of more than 90%, which does not require further puriifcation, with good stability in vitro. hTe association and dissociation constant (KD) of 99mTc-T7 with NCI-H157 tumor cells was 196.1 nM. 99mTc-T7 was mainly metabolism through the internal organs with rapid blood removal. Moreover, the uptake in tumor tissue was signiifcantly higher than the muscle with tumor/muscle ratio of 5.8. In addition, the 99mTc-T7 exhibited a transient accumulation in the lungs. Conclusion hTe 99mTc-T7 could be prepared using a simple method, had high labeling rate and good stability, and could be accumulated at tumor site. hTus, 99mTc-T7 is a poten-tial lung cancer SPECT/CT imaging agent.
