Screening and Establishment of Human Lung Cancer Cell Lines with Organ-speciifc Metastasis Potential
10.3779/j.issn.1009-3419.2014.03.20
- VernacularTitle:器官特异性转移肺癌细胞株的筛选及建立
- Author:
ZHOU QINGHUA
1
,
2
;
ZU LINGLING
;
LI LU
;
CHEN XIAOHE
;
CHEN XIAOFENG
;
LI YANG
;
LIU HONGYU
;
SUN ZHILIN
Author Information
1. 610041成都，四川大学华西医院，四川省肺癌分子重点实验室
2. 300052天津，天津市肺癌转移与肿瘤微环境重点实验室，天津市肺癌研究所，天津医科大学总医院
- Keywords:
Lung neoplasms;
Organ-speciifc metastasis;
Cell lines;
Screening and identiifcation
- From:
Chinese Journal of Lung Cancer
2014;(3):175-182
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Cancer metastasis is not only the malignant marker and characteristics, but also the main cause of failure to cure and lose their life in the patients with lung cancer. Lung cancer metastasis has organ-speciifc characteristics. hTe most common sites of lung cancer metastasis are mediastinal lymph node, brain, bone, liver and adrenal gland. hTe aim of this study is to screen and establish lung cancer cell model with organ-speciifc metastasis potential with human high-metastatic large cell lung cancer cell line L9981 established by our laboratory previously, and to provide cell models for studying the mechanisms and signal regulation of organ-speciifc metastasis of lung cancer. Materials and meth-ods hTe parent lung cancer cell line, L9981-Luc, was inoculated in the armpit of nude mice. hTe live animal imaging system, IVIS-200, was used to detect the lung cancer organ-speciifc metastasis every week. When the organ-speciifc metastasis were established, the nude mices bearing the lung cancer were sacriifced when they became moribund. Under sterile conditions, the organs (mediastinal lymph nodes, lung, spinal column and brain) with lung cancer organ-speciifc metastasis were removed and the metastasized nodules were dissected free of connective tissue and blood clots, and rinsed twice with medium. hTe metas-tasized nodules were ifnely minced using sterile scalpel blades in medium, and the cells were seeded in tissue culture dishes. Then, the cells with organ-specific metastasis potential were reinoculated into the armpit of nude mice, respectively. This processes were repeated to establish the organ-speciifc metastatic sublines of L9981-Luc cell line more than 10 times. Finally, the organ-speciifc metastasis sublines of L9981-Luc were screened and established, which the four cell lines have the charac-teristics only metastasized to brian, lung, bone and mediastinal lymph node. Results A group of organ-speciifc metastasis cell lines which only metastasized to brian, lung, bone and mediastinal lymph node were successfully established through repeat-ing reinoculatation, live animal imaging in nude mice, and screening and identiifcation in vitro. We named the four cell lines as L9981-BoM, L9981-LuM, L9981-BrM and L9981-LnM, respectively. hTe L9981-BoM cell was only metastasized to bone. hTe l9981-LuM cell was only metastasized to lung. hTe L9981-BrM only metastasized to brain. hTe L9981-LnM cell was only metastasized to midiastinal lymph nodes. Conclusions A human large cell lung cancer cell model with bone, lung, brain and lymph node-speciifc metastasis potential was successfully established. It will be helpful to further study the molecular mecha-nisms and signal regulation of lung cancer organ-speciifc metastasis. It will be to also provide reliable cell model for developing new techniques and molecular targeting drugs of inhibiting or reversing lung cancer metastasis.
