Effects of Artesunate on the Invasion of Lung Adenocarcinoma A549 Cells and Expression of ICAM-1 and MMP-9
10.3779/j.issn.1009-3419.2013.11.01
- VernacularTitle:青蒿琥酯对肺癌A549细胞侵袭能力及ICAM-1、MMP-9表达的影响
- Author:
CHEN XIANSHAN
1
;
HAN KUNYUAN
;
CHEN FENGXIA
;
WU CONGMING
;
HUANG WEIYI
Author Information
1. 海南省人民医院胸外科
- Keywords:
Artesunate;
Lung neoplasms;
Invasion;
ICAM-1;
MMP-9
- From:
Chinese Journal of Lung Cancer
2013;(11):567-571
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Artesunate, an anti-malarial drug, elicits an inhibitory effect on pulmonary carcinoma. However, the mechanisms of artesunate activity on pulmonary carcinoma have not been completely elucidated. hTe aim of this study is to investigate the effect of artesunate on the invasion of human lung adenocarcinoma A549 cells. Methods hTe inhibitory effect of artesunate on the proliferation and invasion of A549 cells was determined in vitro by MTT assay and transwell chamber invasion assay, respectively. A nude mouse model of human lung A549 cell xenogratf tumor was established. hTe inhibitory effect of artesunate on the tumor of the mouse model as well as ICAM-1 and MMP-9 protein expressions were determined by Western blot. Results A low dose of artesunate ranging between 1.25μg/L and 5μg/L did not signiifcantly inhibit the proliferation of A549 cells in vitro. By contrast, 1.25μg/L artesunate inhibited the invasion of A549 cells in vitro as determined by transwell chamber invasion assay (96.33±6.41 vs 75.43±4.37, P<0.05). Although 10 mg/kg artesunate did not signiifcantly inhibit A549 xenogratf tumor proliferation (P>0.05), artesunate decreased the ICAM-1 and MMP-9 protein levels in the mouse model (P<0.05). Conclusion Artesunate could inhibit the invasion of human lung adenocarcinoma A549 cells by possibly downregulating ICAM-1 and MMP-9 expressions.
