Expression of PEPT2 mRNA in Lung Tissue of Rats with Pulmonary Fibrosis
10.3779/j.issn.1009-3419.2013.10.08
- VernacularTitle:PEPT2 mRNA在肺纤维化大鼠肺组织中的表达
- Author:
LI LI
1
;
WANG DIANHUA
;
ZHANG XUAN
;
SONG XIN
;
MA XIAOBIAO
;
HU ZAOXIU
Author Information
1. 昆明医科大学第三附属医院云南省肿瘤医院生物治疗中心
- Keywords:
Peptide transporter 2;
Bleomycin;
Pulmonary ifbrosis;
Hydroxyproline
- From:
Chinese Journal of Lung Cancer
2013;(10):541-546
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Pulmonary ifbrosis is a common pathological phenomenon in lung cancer patients atfer chemotherapy or radiotherapy. It is also a key hindrance to the transport of drugs to lung tissue. Peptide trans-porters have become a target of the rational design of peptides and peptide drugs. hTe aim of this study is to investigates the expression of peptide transporter 2 (PEPT2) mRNA in the lungs of rats with bleomycin (BLM)-induced pulmonary ifbrosis. Methods Fitfy healthy adult Sprague-Dawley rats were randomly divided into ifve groups. One group was untreated (control), the second group was injected with normal saline solution (NS), and the three remaining groups were treated with a single dose of bleomycin to induce pulmonary ifbrosis (BLM). Rats from the NS group were killed by exsanguination on day 14. Rats from the BLM group were killed by exsanguination on days 7, 14, and 28. hTe lung samples were observed under light microscopy and the hydroxyproline concentration was determined. hTe expression levels of PEPT2 mRNA were measured by RT-PCR. Results hTe morphological study showed that collagenous ifber proliferated in the lungs of rats injected with BLM, indicating pulmonary ifbrosis. hTis proliferation was apparent at 14 d post-injection and especially at 28 d post-injection. Hydroxyproline levels increased seven days post-injection compared with the control group and NS group, but there was no signiifcant statisti-cal difference (P>0.05). Hydroxyproline levels signiifcantly increased (P<0.05) 14 d and 28 d post-infection. hTe change in the lung tissue pathology coincided with the change in hydroxyproline levels. hTere were no signiifcant changes of pulmonary PEPT2 mRNA expression levels among the different groups (P>0.05). Conclusion PEPT2 is a potential peptide drug target in the treatment of pulmonary ifbrosis, although there were no signiifcant changes of PEPT2 mRNA expression in the lungs of rats with bleomycin-induced pulmonary ifbrosis.
