Identiifcation and Isolation of Cancer Stem Cells from A549 Cells
10.3779/j.issn.1009-3419.2013.08.02
- VernacularTitle:人肺癌A549细胞系肿瘤干细胞的筛选和鉴定
- Author:
XIA HUI
1
;
YU CHANGHAI
;
ZHANG WEN
;
ZHANG BAOSHI
;
ZHAO YINGNAN
;
FANG FANG
Author Information
1. 解放军总医院第一附属医院胸心外科
- Keywords:
A549 cells;
Cancer stem cells;
Lung cancer
- From:
Chinese Journal of Lung Cancer
2013;(8):400-404
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Lung cancer stem cells are the root causes of lung cancer malignant phe-notype and potential therapeutic target, the aim of this study is to isolate and characterize the cancer stem cells in the lung adenoearcinomas cell line A549, so as to provide an experimental basis for further stem cell research. Methods hTe cancer stem cells were isolated from the lung adenoearcinomas cell line A549 using FACS. And the difference of colony formation, cell proliferation and tumorigenicity in vitro were also tested. hTe expression of CD133 and ABCG2 were evaluated by RT-PCR and Western blot. Results hTe percentage of SP cells was 5.93%of A549 and 0.32%of A549 atfer incubation with verapamil. hTe results showed that there were signiifcantly higher expression of CD133 and ABCG2 on SP cells than that of non-SP cells. And the ability of colony formation, cell proliferation and tumorigenicity in SP cell group were remarkably higher than that in non-SP cell group. Conclusion Our results suggested that the cancer stem cells with higher expression of CD133 and ABCG2 can be isolated from the lung adenoearcinomas cell line A549 using FACS and be used in the further research experiments.
