Effects of Methylation of FHIT Gene on it's Protein and mRNA Expression in Non-small Cell Lung Cancer
10.3779/j.issn.1009-3419.2009.07.004
- VernacularTitle:非小细胞肺癌中FHIT基因异常甲基化对其蛋白、mRNA表达的影响
- Author:
LI HONGLI
1
;
ZHANG WEIDONG
;
LI WENTONG
;
YIN CHONGGAO
Author Information
1. 潍坊医学院
- Keywords:
Lung neoplasms;
Methylation;
Methylation-specific PCR;
FHIT gene
- From:
Chinese Journal of Lung Cancer
2009;12(7):760-764
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Fragile histidine triad (FHIT) is a new tumor suppressor gene, whose pro-moter methylation plays an important role in carcinogenesis. The aim of this study is to explore the effects of CpG island meth-ylation on protein and mRNA expression of FHIT in non-small cell lung cancer, and the roles of FHIT gene in pathogenesis of NSCLC. Methods Methylation and protein expression and transcriptional level of FHIT gene were detected by methylation-specific PCK, Western Blot and RT-PCR in 52 tumor tissues and normal tissues of NSCLC. Results Methylation in the tumor samples was detected at 38.46%, whereas it occurred at lower frequencies (7.69%) in the corresponding normal lung tissues. Protein expression frequencies was 28.8% in the tumor samples, whereas it was 88.5% in the corresponding normal lung tissues. MRNA expression frequencies was 51.9% in the rumor samples and 100% in the corresponding normal lung tissues. Conclusion In NSCLC, FHIT gene promoter methylation frequency was significantly increased with decreased expression, suggesting that FHIT promoter methylation plays a role in lung cancer carcinogenesis.
