P120-catenin Isoforms 1A and 3A Differently Affect the Expression of E-cadherin and β-catenin in Lung Cancer Cells
10.3779/j.issn.1009-3419.2009.07.003
- VernacularTitle:肺癌细胞系中p120-catenin亚型1A和亚型3A对E-cadherin和β-catenin的调节功能
- Author:
FANG CHANGQING
1
;
LIU YANG
;
LIU NAN
;
ZHANG YONG
;
WANG ENHUA
Author Information
1. 中国医科大学
- Keywords:
Lung neoplasms;
p120-catenin;
E-cadherin;
Neoplasm invasiveness
- From:
Chinese Journal of Lung Cancer
2009;12(7):741-746
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Different isoforms of p120-catenin (p120ctn), the members of catenin family, are variably expressed in different tissues and cells. These isoforms may confer different properties with respect to cell adhesion and migration, but this concept needs experimental evidence. Methods To more precisely define their biological roles, we stably transfected isoforms 1A and 3A into the lung cancer cell lines A549 and NCI-H460, which had low expression of p120ctn. Using RT-PCR, Western Blot, and Transwell, we testified their impact on the expression ofE-cadherin, β-catenin, and the invasivenessof lung cancer cells. Results Expression of isoform 1A of p120ctn upregulated E-cadherin and β-catenin, as well as the ability of ceils to invasion. In contrast, overexpression of isoform 3A had a smaller influence on invasion. Conclusion P120ctn isoforms 1A and 3A may differently regulate the invasive, adhesive of hing cancer cells through distinct regulation of the expression of E-cadherin and β-catenin.
