Analysis of Differential Gel Electrophoresis of Paclitaxol Resistant and Sensitive Lung Adenocarcinoma Cells' Secretome
10.3779/j.issn.1009-3419.2009.07.001
- VernacularTitle:紫杉醇耐药细胞与敏感细胞分泌蛋白差异凝胶电泳分析
- Author:
SUN QIANGLING
1
;
YANG XIAOHUA
;
LU JING
;
XIE YINYIN
;
CHU TIANQING
;
SHA HUIFANG
Author Information
1. 上海交通大学附属胸科医院
- Keywords:
Differential gel dectrophoresis;
Proteomics;
Secreted protein;
Paclitaxol;
Lung neoplasms
- From:
Chinese Journal of Lung Cancer
2009;12(7):735-740
- CountryChina
- Language:Chinese
-
Abstract:
Background and objective Paclitaxol (PTX) resistance is one of main factors which affect the outcome of chemotherapy of lung adenocarcinoma. The aim of this study is to compare the secreted protein expression profiles between Paditaxol (PTX) resistant and sensitive lung adenocarcinoma cells by proteomic research method, so as to provide evidence of choosing individual chemotherapy drugs in clinical treatment. Methods Total secreted proteins extracted from a PTX sensitive cell line A549 and a PTX resistant cell line A549-Taxol were separated by fluorscent differential gel electrophoresis (DIGE). High quality 2-DE profiles were obtained and analyzed by Decyder 6.5 analysis software to screen differentially expressed protein spots. Those spots were identified by mass spectrometry. Results 2-DE patterns of lung adenocarcinoma cells with high-reso-lution and reproducibility were obtained. 76 significantly differentially expressed protein spots were screened, 19 proteins were identified by mass spectrometry. The identified proteins could be classified into different catogories: metabolic enzyme, extracel-lular matrix (ECM) degradation enzyme, cytokine, signal transducer, cell adhesion, and so on. Conclusion Multiple secreted proteins related to chemoresistance of A549-Taxol cells were identified in this study for the first time. The results presented here would provide dues to identify new serologic chemoresistant biomarkers of NSCLC.
