The role of hexokinase 2 in the metastasis of hepatocellular carcinoma cells
10.3760/cma.j.issn.0253-3766.2016.10.005
- VernacularTitle:己糖激酶2在肝癌转移中的调控作用
- Author:
Xilin GENG
1
;
Weihong LONG
;
Jun HAI
;
Yu ZHANG
;
Wei ZHENG
;
Zhiyong ZHANG
;
Lixue DU
Author Information
1. 陕西省人民医院肝胆外科
- Keywords:
Liver neoplasms;
Hexokinase;
Neoplasm metastasis;
Epithelial-mesenchymal transition
- From:
Chinese Journal of Oncology
2016;38(10):739-742
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory role of HK2 in the metastasis of hepatocellular carcinoma ( HCC) . Methods The protein expressions of HK2 in 73 HCC tumor tissues and paired adjacent non?tumor tissues were evaluated by using immunohistochemical analysis. The scratch wound healing assay and Transwell assay had been used to analyze the migration and invasion of HCC cells with HK2 knockdown. Expressions of epithelial?to?mesenchymal transition ( EMT) markers, such as E?cadherin, ZO?1, N?cadherin and vimentin,in HCC cells with HK2 knockdown were determined by qRT?PCR and Western blot analysis. Results The expression levels of HK2 in tumor tissues and adjacent non?tumor tissues were 5.39±3.40 and 2.16±1.55, respectively. The protein expression of HK2 was significantly higher in tumor tissues compared with adjacent non?tumor tissues of HCC (P<0.05). Knockdown of HK2 in HCC cells decreased the cell motility from 1.00±0.54 to 0.56±0.09 (P<0.05), andknockdown of HK2 in HCC cells decreased the number of invaded cells form 345±42 to 215±34 (P<0.05). The expression of epithelial markers ZO?1 and E?cadherin were up?regulated, while mesenchymal markers vimentin and N?cadherin were down?regulated in HCC cells when HK2 was knockeddown. Conclusions HK2 is up?regulated in HCC and promotes cell motility by stimulating EMT.
