Enhancement of the anticancer activity of cisplatin combined with caffeine and phenobarbital in vitro
- VernacularTitle:咖啡因与苯巴比妥联合对顺铂的体外抗癌增效作用
- Author:
Yanping WANG
1
;
Shulan YUAN
;
Xiaohe CHEN
;
Yan YANG
;
Yi SONG
Author Information
1. 华西医科大学附属第一医院
- From:
Chinese Journal of Lung Cancer
2001;4(3):181-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the enhancing effect of caffeine(CAF) on the anticancer activity of cisplatin on human lung cancer SPC-A-1 cells in vitro and study whether this enhancing effect of caffeine could be inhibited by phenobarbital(PBT). Methods The enhancing effect of combination of caffeine and phenobarbital on the cytotoxicity of DDP on SPC-A-1 was detected by MTT assay. Results No significant cytotoxicity was observed in SPC-A-1 cells treated with 120*!μg／ml CAF alone, 10*!μg／ml PBT alone or both of them. CAF with a concentration of 120*!μg／ml showed significant enhancing effect on the cytotoxicity of DDP on SPC-A-1 (P＜0.05), but PBT of 10*!μg／ml did not (P＞0.05). The combination with CAF of 120*!μg／ml and PBT of 10*!μg／ml could remarkably enhance the cytotoxicity of DDP too (P＜0.01). The enhancing effect of CAF plus PBT on cytotoxicity of DDP was not significantly different from that of CAF (P＞0.05). Conclusion CAF can enhance anticancer activity of DDP in SPC-A-1, which is not antagonized by PBT. The results suggest that as a composition to reduce the side effects of CAF in vitro, PBT may be used as an enhancing agent of chemotherapeutical drugs together with CAF in the treatment of lung cancer.
