Study on the mechanism of binding of tamoxifen and β-lactoglobulin
- VernacularTitle:他莫昔芬与β-乳球蛋白的结合机制研究
- Author:
Jinhua LONG
1
;
Huanhuan SHI
;
Jin CHEN
;
Zhu ZENG
Author Information
- Keywords: beta-lactoglobulin; tamoxifen; static quenching; hydrophobic force; fluorescence quenching; drug carrier; thermomechanical analysis; binding mechanism
- From: Journal of Clinical Medicine in Practice 2023;27(23):12-16,21
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the possibility of β-lactoglobulin(BLG)as carrier of tamoxifen.Methods The binding mechanism of BLG and tamoxifen was studied by spectroscopic technique.Results Tamoxifen would lead to the fluorescence quenching of BLG,and its Kq value was much higher than the maximal Kq value of the dynamic quenching constant[(2.0 × 1010 mol/(L·s)].The quench-ing constant values were different under different pH values,and the values of thermodynamic parame-ters △H and △S were greater than 0.BLG can be combined with tamoxifen to form BLG-tamoxifen complex,and the endothermic process took hydrophobic force as the main force.Tamoxifen and BLG had different binding patterns under acidic and alkaline conditions,which mainly depended on the opening and closing state of the EF ring of BLG.Conclusion BLG has the ability to bind tamoxifen,which shows the potential value of BLG as a carrier of insoluble drugs.
