Expression of P-gp, MRP and p53 and their clinical significance in human lung cancer tissues
- VernacularTitle:P-gp、MRP、p53蛋白在肺癌组织中的表达及其意义
- Author:
Meng RUI
1
;
Longyun LI
;
Lihua LIU
;
Jinglan WANG
;
Zhiyong ZHANG
Author Information
1. Peking Union Medical College Hospital Chinese Academy of Medical Science and Peking Union Medical College
- From:
Chinese Journal of Lung Cancer
2001;4(1):58-62
- CountryChina
- Language:Chinese
-
Abstract:
Objecitve To investigate the clinical roles of P-gp, MRP and p53 expression and their relationship with one another in primary lung cancer. Methods P-gp, MRP and p53 proteins were measured in 31 paraffin-embedded lung cancer tissues and corresponding normal lung tissues by immunohistochemistry(IHC). In addition, flow cytometric analysis of P-gp and p53 was performed in 29 of 31 frozen lung cancer specimens in order to compare the results of the two methods. Results By IHC, positive rates of P-gp, MRP and p53 were 61.3％, 54.8％, 71.0％ respectively in 31 lung cancer cases and there was no expression in all normal lung tissues. P-gp and MRP expressed only in NSCLC. The poorer the cancer cell differentiated, the lower the positive rate of P-gp expression was (P＜0.05). Squamous cell carcinomas showed much higher positive rate of p53 than adenocarcinomas did (P＜0.01). As compared with the non-smokers, staining for p53 was overwhelmingly positive in the smokers (P＜0.05). Neither P-gp nor MRP had significant correlation with p53, however, coexpression of P-gp and MRP had a distinct statistic significance (P＜0.01). By flow cytometry (FCM), the expression rates of P-gp and p53 were 65.5％ and 79.3％ in 29 lung cancer cases. The coincident rates of P-gp and p53 by IHC and FCM were 62.1％, 75.9％ respectively. Conclusion P-gp and MRP overexpression may be involved in the process of lung cancer in co-ordination. Neither P-gp overexpression nor MRP overexpression has significant correlation with p53 alteration.
