Study on molecular pathology in non-small cell lung cancer in Shanghai
- VernacularTitle:非小细胞肺癌分子病理学的研究
- Author:
Kaili XU
1
;
Meilin LIAO
;
Jiaan DING
Author Information
1. Shanghai Cancer Institute
- From:
Chinese Journal of Lung Cancer
2001;4(1):33-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular pathogenesis of non-small cell lung cancer (NSCLC) in Shanghai. Methods About 200 patients with NSCLC from Shanghai urban residents entered in this study. Oncogenes (C-myc, C-erbB2 and EGFR)， tumor suppressor genes (p53 and p15) and some chromosomes instability (3p14, 3p25 and 17p13.3) were determined respectively by DNA slot blot, PCR, PCR-SSCP, and DNA sequences. Results The rate of co-amplification of oncogenes from 174 cases of NSCLC was significantly higher in stage Ⅲ(50.6％, 40／79) and Ⅱ (31.1％, 14／45) than that in stage Ⅰ (16％, 8／50). Total rate of mutations in p53 gene exons was 49.2％(31／63), while the rate of exon 8 point mutation was the highest. The rate of loss of homozygosity of p15 gene was higher in stage Ⅲ (61.4％, 43／70) than that in stage Ⅰ (34.6％, 18／52) (P＜0.05). 40％(8／20) of loss of heterozygosity at 17p13.3 and 66.7％(108／162) at 3p14 or 3p25 were observed. The rate of co-deletion on 3p14 and 3p25 of adenocarcinoma was higher than that of squamous cell carcinoma. Conclusion The results suggest that the features of molecular pathogenesis in this series include: (a) 3p deletion in precancerous lesion; (b) p53 mutation and 17p13.3 deletion in carcinoma in situ; (c) amplification of oncogenes for C-myc, c-erbB2, EGFR and deletion of p15 gene in invasion and metastasis carcinoma of the lung.
