Anti-tumor effect of newcastle disease virus (NDV) on human lung adenocarcinoma xenografts
- VernacularTitle:新城疫鸡瘟病毒对裸小鼠人肺腺癌移植瘤的抑瘤作用及机制的研究
- Author:
Yingzhun CHEN
1
;
Quanyu XU
;
Lifeng WANG
;
Gang ZHAO
;
Shuxiang LI
Author Information
1. Ha'rbin Medical University
- From:
Chinese Journal of Lung Cancer
2001;4(1):29-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the anti-tumor effects of NDV and two genes of virus(HN and F) on athymic mice with human adenocarcinoma xenografts, and to investigate the mechanisms of its oncolytic role. Methods The experimental model of lung adenocarcinoma xenograft was established. The two experimental groups of athymic mice were given intratumoral injections of NDV and plasmids only once, and compared with PBS controls in the same time. Measure the volume of tumors for 5 weeks and make a curve of the volume. These mice were killed after 5 weeks, and the weight of the tumors was measured. The histological and ultrastructral changes were observed by electromicroscope and microscope. Results After one injection of live NDV and plasmids, the tumor growth was significantly suppressed (The median inhibitory rate was 71.62％ and 79.40％ respectively). The median weight of tumor of mice treated with NDV was remarkably lower than that of mice treated with PBS, and that of the mice treated with plasmids (P＜0.01). 14％ of the control group had liver and lung metastasis of the tumor, but no metastasis was found in the experimental groups. A great quantity of NDV viron budding was found in the NDV group. Conclusion NDV could replicate in human lung adenocarcinoma xenografts, leading directly to a potent anti-tumor effect after one injection of live NDV. During the oncolytic process, the gene HN and gene F may play an important role.
