Analysis of Polymethoxyflavonoids in Citri Reticulatae Pericarpium by HPLC-Q-TOF-MS
10.14148/j.issn.1672-0482.2024.1223
- VernacularTitle:基于HPLC-Q-TOF-MS分析陈皮中多甲氧基黄酮部位的化学成分
- Author:
Huiling TANG
1
;
Jingyan ZHANG
;
Bingyan TAN
;
Yin SUN
;
Jin-Yao SONG
;
Yi-Yang ZHANG
;
Xinru LI
;
An KANG
Author Information
1. 江苏食品药品职业技术学院药学院,江苏 淮安 223001
- Keywords:
Citri Reticulatae Pericarpium;
polymethoxyflavonoid;
HPLC-Q-TOF-MS
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2024;40(11):1223-1233
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To enrich the polymethoxyflavonoid-rich fraction from Citri Reticulatae Pericarpium using D101 macro-porous resin,and further to analyse the chemical constituents using High-Performance Liquid Chromatography Quadrupole-Time-of-Flight Mass Spectrometry(HPLC-Q-TOF-MS).METHODS Citri Reticulatae Pericarpium extract was adsorbed on D101 macro-porous resin and then eluted with different concentrations of ethanol to enrich the polymethoxyflavonoid-rich fraction;the content of the major flavonoid components was determined by high-performance liquid chromatography(HPLC);and the components were further an-alysed by HPLC-Q-TOF-MS.RESULTS The established HPLC method for the simultaneous determination of seven flavonoids in Citri Reticulatae Pericarpium extract was accurate and reliable.The contents of compounds such as nobiletin and tangeritin were signifi-cantly increased in the polymethoxyflavonoid-rich fraction.Using high resolution mass spectrometry(HRMS),70 and 60 compounds were identified from the Citri Reticulatae Pericarpium extract and the polymethoxyflavonoid-rich fraction,respectively,with 53 polyme-thoxyflavonoids being detected in these two extracts.CONCLUSION The results of this study provide an experimental basis for fur-ther identification of the pharmacological substance basis of the polymethoxyflavonoids in Citri Reticulatae Pericarpium and the estab-lishment of quality control methods.
