Relationships of serum microRNA-20a-5p and microRNA-128-3p with cranial nerve development in children with hypoxic-ischemic encephalopathy
- VernacularTitle:血清微小RNA-20a-5p、微小RNA-128-3p与缺氧缺血性脑病患儿脑神经发育的关系
- Author:
Ruiwu ZHU
1
;
Xiaojie GUO
;
Qing ZHANG
Author Information
- Keywords: hypoxic-ischemic encephalopathy; premature infants; microRNA-20a-5p; mi-croRNA-128-3p; cranial nerve development
- From: Journal of Clinical Medicine in Practice 2023;27(22):50-54
- CountryChina
- Language:Chinese
- Abstract: Objective To analyze the relationships of expressions of serum microRNA(miR)-20a-5p and miR-128-3p with cranial nerve development in the premature infants with hypoxic-ischemic en-cephalopathy(HIE).Methods A total of 95 HIE premature infants were selected as HIE group,and according to the severity of HIE,they were divided into mild group(n=27),moderate group(n=46)and severe group(n=22);another 95 premature infants without HIE in the same period were se-lected as control group.Apgar score and the Neonatal Behavioral Neurological Assessment(NBNA)score were performed in both groups;the expression levels of miR-20a-5p and miR-128-3p in serum were detected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR);Spearman method was used to analyze the correlations of serum miR-20a-5p and miR-128-3p levels with Apgar score and NBNA score;the values of serum miR-20a-5p and miR-128-3p levels in diagnosing HIE in premature infants were analyzed by receiver operating characteristic(ROC)curve.Results Compared with the control group,the levels of serum miR-20a-5p and miR-128-3p in the HIE group increased sig-nificantly,while the Apgar score and NBNA score decreased significantly(P＜0.05).In the mild,moderate,and severe groups,the levels of serum miR-20a-5p and miR-128-3p increased gradually,while the Apgar score and NBNA score decreased gradually,with the significant between-group differences(P＜0.05).Spearman analysis results showed that the level of serum miR-20a-5p was negatively correlated with the Apgar score and NBNA score(r=-0.659,-0.548,P＜0.001),and the level of serum miR-128-3p was negatively correlated with the Apgar score and NBNA score(r=-0.582,-0.499,P＜0.001).The combined prediction of serum miR-20a-5p and miR-128-3p for HIE in premature infants had an area under the curve(AUC)of 0.920,with a sensitivity of 78.95％and a specificity of 97.89％.The predictive efficacy of the combined model was superior to that of miR-20a-5p and miR-128-3p alone(P＜0.05).Conclusion The serum levels of miR-20a-5p and miR-128-3p increase in the premature infants with HIE,and are closely related to the severity of the disease and the development of brain nerves.The combined detection of miR-20a-5p and miR-128-3p has good predictive value for the occurrence of HIE in premature infants.
