Experimental study on apoptosis of gastric cancer cell line SGC-7901 induced by coix seed oil
10.7619/jcmp.201908001
- VernacularTitle:薏苡仁油诱导胃癌细胞SGC-7901凋亡的实验研究
- Author:
Guozhuang SUN
1
;
Youjun CAO
;
Haiyan MAO
;
Heng LUO
;
Zidu HE
;
Guimei KONG
Author Information
1. 扬州大学医学院 转化医学研究院
- Keywords:
coix seed oil;
gastric cancer;
protein arginine methyltransferase 5;
migration
- From:
Journal of Clinical Medicine in Practice
2019;23(8):1-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible mechanism of coix seed in inducing apoptosis of gastric cancer cells (SGC-7901). Methods Different concentrations of coix seed oil (2, 4, 8 mg/mL) were applied to SGC-7901 cells. MTT assay was used to detect the effect of drugs on cell proliferation, and flow cytometry for drug-induced cell apoptosis, scratch test for cell migration inhibited by coix seed oil, Transwell chamber for drug-inhibited cell invasion, and Western blot for expression of related proteins PRMT5, PI3 K and AKT. Results The results of MTT showed that 2, 4 mg/mL of coix seed oil could significantly inhibit the proliferation of SGC-7901 cells, and the inhibition rate of 2 mg/mL was (30. 02 ± 1. 56) ％, which showed significant difference compared to the control group (P ＜ 0. 01). The results of flow cytometry showed that the apoptosis rates of coix seed oil cells at concentrations of 2 and 4 mg/mL were (16. 25 ± 2. 54) ％, (12. 60 ± 1. 12) ％, respectively, and which showed a significant difference compared with (2. 0 ± 1. 22) ％ in the control group (P ＜ 0. 01). The results of cell scratch test showed that the migration of SGC-7901 cells treated with 2, 4 mg/mL of coix seed oil had significant difference when compared to control group (P ＜0. 01). The results of invasion experiments showed that 2, 4 mg/mL of coix seed oil could significantly inhibit cell invasion, and the number of cell invasion was (134. 00 ± 2. 86), (167. 00 ±0. 99), respectively, which showed significant difference compared to (268. 00 ± 2. 05) in the control group (P ＜ 0. 01). The migration number in 8 mg/mL coix seed oil group was (167 ± 0. 99), a significant difference was observed when compared to the control group (P ＜ 0. 05). Western blot analysis showed that different concentrations of coix seed oil could significantly down-regulate the expression of PRMT5, PI3 K and AKT in SGC-7901 cells. Conclusion Coix seed oil can significantly inhibit the proliferation, migration and invasion of gastric cancer SGC-7901 cells, its possible mechanism is to down-regulate the signal pathway of PRMT5-PI3 K/AKT to inhibit the activation of various anti-apoptotic molecules, induce apoptosis and suppress invasion and metastasis of gastric cancer SGC-7901 cells by down-regulating the PRMT5-PI3 K/AKT signaling pathway.
